SKU: 10399060673

Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit

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Description

Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA KitThe Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit measures BRAK in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BRAK antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Mouse BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit measures BRAK in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BRAK antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with BRAK bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of BRAK in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 10399060673

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4.8 ★★★★★
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Bruce Judisch
Houston, US
★★★★★ 5
Concise, well researched and objective; a great homeschool resource
This is a great read; sufficiently detailed to be worth the time spent, but not so bogged down in minutia that you find yourself wondering how long the chapter is going to go on and glancing ahead to see if it ends with a quiz. Particularly notable is the prose: flowing, easy to read, a comfortable narrative. You get a sense of the author relaxed in an easy chair, his audience gathered about, attentive ears regaled with true tales of valor, intrigue, and conquest. But you will find no glorification of war between these covers; rather a well-balanced rendering of man at his best within his worst. “Great Battles” is well researched, factual, and untainted with retroactive speculation and subjective parochialism that pervade some of today’s ‘historical’ accounts. In short, a valuable addition to any homeschool or military historian’s library; highly recommended.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 27, 2014
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Verified Purchase
Sarah
Omaha, US
★★★★★ 5
FINALLY!
Format: Paperback
I was pleased to finally find an historical series written for young boys that neither "cartoonizes" war nor adopts a satirical, demeaning tone. Rather, these books depict the true sacrifices of the men involved in these conflicts. The facts are the facts. There is no politically correct hogwash clouding the child's view of the event. These are more mature historical articles about pivotal events no longer discussed in our public school! These books glorify the ideals scrapped by post-modernists -- bravery, chivalry, and heroism -- ideals that once defined masculinity and limited its so-called "toxicity." As a mother raising masculine gentlemen, I will continue purchasing these fine books! Thanks, Mr. Giorello! Keep writing!
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Reviewed in the United States on October 3, 2019
C
Verified Purchase
CGW
Grantham, US
★★★★★ 5
Very useful
Format: Paperback
My grandson likes history. This book has proven to be a good starting point for him to then delve deeper into the various wars and battles.
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Reviewed in the United States on November 7, 2025
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James A Doherty III
Massapequa, US
★★★★★ 5
I like it!
Format: Paperback
My 12 yr. old son loved this series.
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Reviewed in the United States on April 2, 2025
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Amazon Customer
West Palm Beach, US
★★★★★ 5
Perfect choice for 9yr old
Format: Paperback
Grand daughter, 9 yrs,asked for this book. She really likes this author.
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Reviewed in the United States on January 1, 2026

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