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Description
Rabbit Bcl-10 (B-cell Lymphoma/Leukemia 10) ELIS Kit ELISA KitThe Rabbit Bcl 10 (B cell Lymphoma Leukemia 10) ELIS kit ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit Bcl 10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit Bcl 10 and Avidin Horseradish Peroxidase (HRP) conjugate are added
The Rabbit Bcl-10 (B-cell Lymphoma/Leukemia 10) ELIS kit ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit Bcl-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit Bcl-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit Bcl-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit Bcl-10. You can calculate the concentration of Rabbit Bcl-10 in the samples by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
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