SKU: 16167664008

Porcine IL-1Beta (Interleukin 1 Beta) ELISA Kit

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Description

Porcine IL-1Beta (Interleukin 1 Beta) ELISA KitThe Porcine IL 1Beta (Interleukin 1 Beta) ELISA Kit measures IL 1Beta in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Porcine IL-1Beta (Interleukin 1 Beta) ELISA Kit measures IL-1Beta in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1β antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1β bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1β in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 16167664008

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4.5 ★★★★★
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Leonard G.
Natrona Heights, US
★★★★★ 3
Lasted less than 2 weeks for our Heeler.
Pattern Name: Duck
Wish it had more armor guard for tough tethers. Lasted only 2 weeks & that is a long time. She has 8 different chew toys of this type.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
H
Verified Purchase
honest opinions
New York, US
★★★★★ 5
Durable- well made
Pattern Name: Donkey
Very durable. Our dog loves this toy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 17, 2026
B
Verified Purchase
Barb
Port Orchard, US
★★★★★ 1
Crinkley Paper Should Not be Ingested
Pattern Name: Donkey, Pattern Name: Donkey
This thing was cute as can be, and for awhile my dogs got some good play time out of it. But I was somewhat mortified when I saw torn up pieces of the "crinkley paper" all over my living room carpet. It is like a crunchy Saran Wrap, definitely not something a dog should be potentially ingesting. They can say the toy is tough and durable, but it ultimately is not, so it matters what is inside it. I really can't believe they would put something like that inside a toy. As soon as I saw it, I immediately threw the toy in the garbage. All dog toys have something inside them that is pretty non-digestible, but this plastic seemed particularly wreckless to me. So cute or not, I cannot recommend this toy and will not buy from this manufacturer again.
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Reviewed in the United States on March 20, 2026
B
Verified Purchase
Beverly B Gutshall
Phoenix, US
★★★★★ 5
Tough toy
Color: Orange
This toy has a name in our house.its very tough takes on alot of tug of war but sometimes the seams tear so I have to keep one on hand at all times.tougher than other toys I have purchased
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Reviewed in the United States on April 28, 2026
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Verified Purchase
Nancy Tasker
Carnegie, US
★★★★★ 1
Not for aggressive chewers!!!
Color: Auburn, Color: Auburn
Advertised for aggressive chewer and less than a half a day with him playing and its destroyed. I had to take away from him and cut off ears, nose and tail. Hes only a chihuahua puppy. Definitely not as advertised
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Reviewed in the United States on June 8, 2026

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