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Description
Mouse IL-4 (Interleukin 4) ELISA KitThe Mouse (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in Mouse samples. The plate has been pre coated with Mouse IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Mouse (IL-4) Interleukin 4 ELISA Kit measures Interleukin 4 in Mouse samples. The plate has been pre-coated with Mouse IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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