SKU: 25455607878

Human OPN (Osteopontin) QuickTest ELISA Kit

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Description

Human OPN (Osteopontin) QuickTest ELISA KitThe Human OPN (Osteopontin) QuickTest ELISA Kit measures OPN in Human Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual wells.

The Human OPN (Osteopontin) QuickTest ELISA Kit measures OPN in Human Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains OPN, a capture antibody-OPN-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of OPN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 25455607878

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4.1 ★★★★★
Based on 16 reviews
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barb belshe
Los Angeles, US
★★★★★ 5
Withstands very aggressive chewing
Color: Blue
We have a very active and aggressive chewer. Items identified for aggressive chewers are “Gone in 60 Seconds”. This toy is amazing. It has withstood Jake’s many attempts to destroy it and is one of his favorites! It is not hard like acrylic but is hard rubber with some give decreasing risk of fractured teeth. Best toy yet!!!
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Reviewed in the United States on May 8, 2026
C
Verified Purchase
Candace McCullough
Los Angeles, US
★★★★★ 5
Great chew toy
Color: Blue
I bought this almost a year go. My dog is still chewing on it! Nothing last this long with her. She is a 100lb Rottweiler that is a strong chewer.
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Reviewed in the United States on June 12, 2026
D
Verified Purchase
Dawn Clegg
Birmingham, US
★★★★★ 4
Well loved
Color: Blue, Color: Blue
We just got this sweet girl from the shelter a few days ago. She's had this toy for 2 days and already chomped off one of the legs. She does have other toys so she doesn't play with this one all the time but she does like it a lot! It's very hard and durable on the ends. But as you can see, it's not indestructible. She carries this little guy around the house like they're BFFs. It's super adorable.
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Reviewed in the United States on May 21, 2026
G
Verified Purchase
Greg B.
Houston, US
★★★★★ 5
Durable and a favorite toy
My 7 month old Black Lab loves this bone. She is a chewer and has gone to town on the three ends. They are roughed up a bit but holding up better than expected. Highly recommend for dogs that love to chew.
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Reviewed in the United States on June 6, 2026
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Verified Purchase
Lea
Alexandria, US
★★★★★ 5
Perfect for aggressive chewers
I have a 2 year old pittie and she chows thru bones in minutes, even those reviewed as "long lasting". This thing is still going strong after 9 months. She was not initially interested, had to spread peanut butter on one end, one time, and now it is her go-to chew toy. Looks a little mangled after all this time but she's not even 75% thru it. Highly recommend, no stomach issues.
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Reviewed in the United States on June 7, 2026

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