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Description
Human sNOX2-dp (Soluble NOX2-derived peptide) ELISA KitThe Human sNOX2 dp (Soluble NOX2 derived peptide) ELISA Kit measures sNOX2 dp in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti sNOX2 dp antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
The Human sNOX2-dp (Soluble NOX2-derived peptide) ELISA Kit measures sNOX2-dp in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti sNOX2-dp antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with sNOX2-dp bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of sNOX2-dp in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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