SKU: 33735514872

Bovine PG (Progesterone) ELISA Kit

Sale price$234.00 Regular price$260.00
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Ships within 48 hours · Estimated delivery Jul 27 - Aug 1

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Description

Bovine PG (Progesterone) ELISA KitThe Bovine PG (Progesterone) ELISA Kit measures Pg in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Pg. Pg in the sample or standard competes with a fixed amount of biotin labeled Pg for sites on a pre coated antibody specific to Pg. Free components are washed off. After adding HRP

The Bovine PG (Progesterone) ELISA Kit measures Pg in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Pg. Pg in the sample or standard competes with a fixed amount of biotin-labeled Pg for sites on a pre-coated antibody specific to Pg. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Pg in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 33735514872

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