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Description
Canine/Feline Clusterin ELISAThe Canine Feline Clusterin ELISA is an enzyme immunoassay for the quantitative determination of canine feline clusterin in urine, serum and plasma. In the Canine Feline Clusterin ELISA, standards, quality controls and samples are incubated in microplate wells pre coated with specific anti canine feline clusterin antibody. After 60 minutes incubation and washing, biotin labelled anti canine feline clusterin antibody is added and incubated with
The Canine/Feline Clusterin ELISA is an enzyme immunoassay for the quantitative determination of canine/feline clusterin in urine, serum and plasma.
In the Canine/Feline Clusterin ELISA, standards, quality controls and samples are incubated in microplate wells pre-coated with specific anti-canine/feline clusterin antibody. After 60 minutes incubation and washing, biotin labelled anti-canine/feline clusterin antibody is added and incubated with captured clusterin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of clusterin. A standard curve is constructed by plotting absorbance values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
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