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Description
Rat LR (Leptin Receptor) ELISA KitThe Rat (LR) Leptin Receptor ELISA Kit measures Leptin Receptor in Rat samples. The plate has been pre coated with Rat LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (LR) Leptin Receptor ELISA Kit measures Leptin Receptor in Rat samples. The plate has been pre-coated with Rat LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat LEPR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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