SKU: 65573509393

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 15 - Jul 20

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Description

8-OHdG (8-Hydroxydeoxyguanosine) ELISA KitThe 8 OHdG (8 Hydroxydeoxyguanosine) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with 8 OHdG. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to 8 OHdG. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The

The 8-OHdG (8-Hydroxydeoxyguanosine) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 65573509393

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