SKU: 69684715447

Rat CERAMIDE (Ceramide) ELISA Kit

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Description

Rat CERAMIDE (Ceramide) ELISA KitThe Rat CERAMIDE (Ceramide) ELISA Kit measures Ceramide in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Ceramide. Ceramide in the sample or standard competes with a fixed amount of biotin labeled Ceramide for sites on a pre coated antibody specific to Ceramide. Free components are washed

The Rat CERAMIDE (Ceramide) ELISA Kit measures Ceramide in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Ceramide. Ceramide in the sample or standard competes with a fixed amount of biotin-labeled Ceramide for sites on a pre-coated antibody specific to Ceramide. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Ceramide in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 69684715447

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4.3 ★★★★★
Based on 24 reviews
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Woodrow Carter
Phoenix, US
★★★★★ 5
Nice and comfortable shoes at a good price at a good price
Size: 9, Color: Cognac
Willow made you had a good price, attractive and comfortable a attractive and comfortable at a good price
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Reviewed in the United States on June 5, 2025
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Verified Purchase
GENE LECKNESS
San Leandro, US
★★★★★ 5
Beyond my expectations
Size: 10 Wide, Color: Mocha Grainy Leather
Really nice shoes. Wide enough and comfortable I’m keeping these.
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Reviewed in the United States on May 14, 2026
M
Verified Purchase
Mike
New York, US
★★★★★ 5
Good Fit Out of the Box
Size: 9, Color: Black
The shoes came with the correct size and is a good fit. They were originally pretty stiff, but are much better now after breaking them in. They look good. but as other's have said, they are made of synthetic leather and in my opinion have kind of a plastic look to them. The quality is good with ridged rubbber soles. I thought they were kind of expensive, but then again I haven't purchased these type of shoes in a while. A family member told me that if they were real leather, they would have been a lot more expensive. For what the shoes are I would recommend the purchase.
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Reviewed in the United States on February 11, 2025
M
Verified Purchase
Mar Ronquillo
Grantham, US
★★★★★ 3
I wanted to love these
Size: 9, Color: Black Leather
These are very good looking in person, especially the buckles and the pop of red on black. Alas, I have another pair of Marc Joseph shoes to compare comfort and fit, and this pair simply hurts my feet.
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Reviewed in the United States on May 31, 2026
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Verified Purchase
Wei
Belleville, US
★★★★★ 5
Fitness
Size: 9, Color: Black Leather
Comfortable
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Reviewed in the United States on June 5, 2026

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