Human GPR116 (G-protein coupled receptor 116) ELISA Kit

Human GPR116 (G-protein coupled receptor 116) ELISA KitThe Human GPR116 (G protein coupled receptor 116) ELISA Kit measures GPR116 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GPR116 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human GPR116 (G-protein coupled receptor 116) ELISA Kit measures GPR116 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GPR116 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with GPR116 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of GPR116 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.