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Description
Human PRKA3 (A Kinase Anchor Protein 3) ELISA KitThe Human (PRKA3) A Kinase Anchor Protein 3 ELISA Kit measures A kinase anchor protein 3 in samples. The plate has been pre coated with Human PRKA3 antibody. PRKA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRKA3 Antibody is added and binds to PRKA3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PRKA3 antibody. After incubation unbound Streptavidin HRP is washed
The Human (PRKA3) A Kinase Anchor Protein 3 ELISA Kit measures A kinase anchor protein 3 in samples. The plate has been pre-coated with Human PRKA3 antibody. PRKA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRKA3 Antibody is added and binds to PRKA3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRKA3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PRKA3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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