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Description
Rat HSP72 (Heat Shock Protein 72) ELISA KitThe Rat (HSP72) Heat Shock Protein 72 ELISA Kit measures Heat Shock Protein 72 in Rat samples. The plate has been pre coated with Rat HSPA2 antibody. HSPA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSPA2 Antibody is added and binds to HSPA2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPA2 antibody. After incubation unbound Streptavidin HRP is washed away
The Rat (HSP72) Heat Shock Protein 72 ELISA Kit measures Heat Shock Protein 72 in Rat samples. The plate has been pre-coated with Rat HSPA2 antibody. HSPA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSPA2 Antibody is added and binds to HSPA2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HSPA2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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