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Description
Human TSC22 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transforming Growth Factor Beta Stimulated Protein Clone 22 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TSC22 domain family protein 1, also known as transforming growth factor-β-stimulated protein 22 (TSC22), is a protein encoded by the TSC22D1 gene. TSC22 encodes a transcription factor that belongs to a large family of early response genes. TSC22D1 forms homodimers through its conserved leucine zipper structure and heterodimerizes with TSC22D4. TSC22D1 exhibits transcriptional repression activity. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.1 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Finally, a framework that makes sense of AI's impact on knowledge work
Format: Kindle
Most books about AI focus on task automation and productivity gains. Reshuffle does something different: it explains how AI restructures entire systems through three constraints: tasks, coordination, and risk.
For someone working in the language services industry, this book was revelatory. It helped me understand why so many conversations about AI and translation feel misdirected. We debate whether AI will replace translators when the real question is: how will AI reshuffle who creates value in language services?
Choudary's central insight is that when AI removes old constraints (like scarcity of expertise), value doesn't disappear. It migrates to new coordination and risk management challenges. This applies across all knowledge professions, not just translation.
Section 2 on knowledge work is particularly strong. It shows that lawyers, consultants, accountants, and translators are all experiencing the same fundamental transformation. We're not uniquely vulnerable; we're part of a larger reshuffling of how knowledge creates value.
If you're trying to position yourself or your organization for what's coming, this book offers the clearest framework I've found. It's not about having better AI tools. It's about understanding where value pools are forming in the new system.
Recommended for anyone in knowledge work who wants to move beyond surface-level AI discussions.
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Reviewed in the United States on January 25, 2026
★★★★★ 5
Not like any other how-to book on AI--Eric Swanson's Review
Format: Kindle
Reshuffle is not another “how to use AI” guide. It’s a powerful, big-picture look at how AI is reshaping the very foundations of the knowledge economy. Sangeet doesn’t just explore tools—he reveals the tectonic shifts in how knowledge is created, distributed, and valued. Most people use AI to improve old systems; this book shows why the winners will be those who understand and adapt to entirely new ones. Using powerful examples from history, like the bar code, container boxes and the Maginot Line, Sangeet creates powerful frames for new ways of thinking. Insightful, clear, and compelling, Reshuffle is essential reading for anyone who wants to lead in the age of AI
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Reviewed in the United States on July 23, 2025
★★★★★ 5
Would your card still be in the deck after the AI reshuffle?
Format: Paperback
AI’s impact on knowledge workers, and on enterprises, is immense. “Good enough” and inexpensive answers now abound, and the premium once commanded by knowledge workers seems to be slipping away. Enterprises are pinning their hopes on AI-driven efficiencies to stay competitive and relevant. Emotions surrounding this technological breakthrough range from doom and gloom to glee and hope.
Sangeet’s Reshuffle helps build a mental model to understand, navigate, and survive this change, and even thrive in it. It’s a refreshing departure from the usual first-order effects and fallacies that dominate social and print media.
For knowledge workers, staying relevant is becoming increasingly difficult, especially as the very definition of “relevance” evolves. Simply acquiring AI skills may not suffice if the underlying value of those skills has shifted. Judgment, systems thinking, and coordination will become more valuable. Remaining well-paid and autonomous will require protecting and growing contextual and economic value within this transformed system. Simple, but not easy.
At the enterprise level, applying AI for task-based efficiencies in one area often shifts constraints elsewhere. Using systems thinking and positioning AI as the engine, not merely a tool, for innovation and coordination across the value chain will give enterprises a fighting chance to stay competitive.
While the metaphorical pie may grow, simply “playing the same game better” won’t earn you a proportional share of it. Existing systems will be unbundled and re-bundled into offerings that solve emerging constraints. Coordinating across the value chain and taking responsibility for delivering customer outcomes will be key to unlocking outsized gains.
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Reviewed in the United States on October 29, 2025
★★★★★ 5
A great addition to my Kindle library and a candidate to our Best Book Picks of 2025.
Format: Kindle
In setting the scene, Sangeet reminds us that, in the 1960s, Singapore was a struggling port city with limited natural resources and a rather tenuous future. It's hard to imagine but true. A strategic location in South East Asia. But such location meant little if it could not draw talent and capital to develop the infrastructure needed to grow, and here a deceptively simple and modular invention helped - the shipping container.
Harvard Professor Carliss Y. Baldwin, in her book Design Rules, shared with us how technology shapes organisations, indeed entire industries and societal structures, and so, as we envision and put a technology to use, who decide how organisations are shaped, who governs them, and where power and agency lies.
Yet AI is not just any other technology. We are not in full control of the technology and its power to learn, re-shape itself, and its impact on the nature of work therefore extends well beyond the individual using AI tools. This is where Sangeet takes us, into a hugely relevant and timely discussion of how AI presents immense opportunities as well as grave risks to the knowledge economy, as we know it today.
The questions raised are profound: among these...
- How would power shift from the current ways of work we are accustomed to, towards autonomous networks that make decisions and learn on their own (and faster than us)?
- Which organizational models best capture the shifts towards AI-supported value creation? and what path could such a transition follow?
- How would these impact the opportunities and risks for collaboration, within and beyond the enterprise?
A whole chapter is dedicated to strategy, and deservedly so. AI in itself does not provide a competitive advantage. Let’s not rush to appoint a Chief AI Officers or draw-up a so-called AI-strategy, for what is essentially a set of widely distributed and accessible technologies. We need a business strategy that acknowledges the deep impacts AI is and will continue to make. Before we rush to layer AI on top of org. processes and models that have served us in previous generations, let’s take an ecosystem-wide view and ask - where are we now? What is fundamentally changing, and Where can we harness its trends towards an advantage?
Having read Sangeet's book, my advice is this - seize the opportunity, invite others to the conversation and be open to new forms of power and control, as the organisations that win tomorrow are already experimenting in doing things differently today.
A great addition to my Kindle library and a candidate to our Best Book Picks of 2025.
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Reviewed in the United States on August 15, 2025
★★★★★ 5
Amazing Study Guide for Passing the Security+ Exam Quickly!
Format: Paperback
I recently used the CompTIA Security+ Get Certified Get Ahead: SY0-701 Study Guide to prepare for my Security+ exam, and I passed in just two weeks—a huge accomplishment for me! Here’s why this study guide was so helpful:
1. Clear and Concise Content
The book is well-organized and breaks down the material in a way that’s easy to understand, even if you’re new to security concepts. It covers everything you need for the SY0-701 exam, from network security to risk management and cryptography, in a clear, digestible format. It’s not overwhelming, and the explanations are straightforward.
2. Focused on Exam Objectives
What I really appreciated was how the guide is directly aligned with the exam objectives. It helps you focus on what’s important and ensures you're prepared for the exact topics that will be on the test. I didn’t waste time studying irrelevant material, and I felt confident on exam day.
3. Practice Questions and Exam Tips
The practice questions at the end of each chapter were incredibly useful. They gave me a feel for the types of questions I’d encounter on the actual exam, and reviewing the answers helped me solidify my understanding. The exam tips and tricks scattered throughout the book also helped me refine my test-taking strategies.
4. Comprehensive Coverage with Easy-to-Understand Examples
This guide provides not only the theory but also real-world examples that helped make abstract concepts easier to grasp. The examples helped me see how the material applied in actual IT environments, which made it easier to remember.
5. Great for Quick Study Sessions
I had a tight two-week timeline to study, and this guide was perfect for that. The chapters are concise enough to work through in short study sessions, and the material is presented in a way that’s easy to retain. I was able to go through the entire book and still have time to review key concepts before my exam.
Final Thoughts
If you’re looking to pass your CompTIA Security+ SY0-701 exam in a short amount of time, this study guide is an excellent resource. It helped me prepare effectively, and I passed the exam with confidence in just two weeks! Highly recommend it for anyone looking to get certified and get ahead in their career.
Pros:
Clear, concise explanations aligned with exam objectives
Practice questions and exam tips to reinforce learning
Real-world examples that make concepts easier to understand
Perfect for fast, focused study sessions
Helps you stay on track for the Security+ exam
Con:
Some sections could benefit from deeper dives for advanced learners, but it’s perfect for exam preparation
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Reviewed in the United States on December 31, 2024