SKU: 63137015094

UA-Blue SEAP Detection Kit

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Description

UA-Blue SEAP Detection KitProduct Specification Synonyms Alkaline phosphatase detection reagent Stability & Storage Store reagents at 20C or below, protected from light . Shelf Life: 12 months when stored as recommended. For research use only. Background Secreted Alkaline Phosphatase (SEAP) is widely used in reporter gene analysis studies. Its advantage is that it only requires the analysis of cell culture supernatant, without the need to lyse cells. Moreover, SEAP is

Product Specification


Synonyms Alkaline phosphatase detection reagent
Stability & Storage

Store reagents at -20°or below, protected from light . Shelf Life: 12 months when stored as recommended. For research use only.

Background

Secreted Alkaline Phosphatase (SEAP) is widely used in reporter gene analysis studies. Its advantage is that it only requires the analysis of cell culture supernatant, without the need to lyse cells. Moreover, SEAP is relatively heat-resistant and has a high tolerance for specific alkaline phosphatase inhibitors. Therefore, samples can be subjected to specific pre-treatment to inactivate endogenous alkaline phosphatase and eliminate interference.

The UA-Blue SEAP Detection Kit is a homogeneous ready-for-use reagent that completes the detection through the simple steps of “add-mix-detect.” This kit is used to detect alkaline phosphatase activity in the culture medium of cells transfected with the SEAP reporter gene. There is no need to lyse the cells, which can be further cultured or used for other multiplex analyses. Compared with other similar products, the detection sensitivity is at least one time higher, and the reaction time is shortened by at least one time. It is particularly suitable for the analysis of cells with low enzyme activity and the screening of inhibitory compounds.

Components

Size

Buffer (40x)

Substrate (250x)

Tests in 96-well plates

Tests in 384-well plates

2500 tests

5x 2.5 ml

5x 400 ml

2,500

12,500

25000 tests

5x 25 ml

5 x 4ml

25,000

125,000


Protocol

1). Cell Seeding & Preparation

Plate cells expressing SEAP reporter gene in 96-well or 384-well plates.

If using FBS-containing medium: Heat-inactivate FBS at 56°C for 30 min to eliminate endogenous alkaline phosphatase (AP) activity.

 

2). Cell Treatment & Stimulation

Treat cells with test compounds and stimulate reporter gene expression as required.

 

3). Sample Collection & Pre-Treatment

Collect cell culture supernatant at the desired timepoint post-stimulation. For high endogenous AP background: Heat samples at 65°C for 5-10 min to inactivate interfering phosphatases (optional).

Note: The QT-Blue™ SEAP Assay Kit reagent suppresses low levels of endogenous AP without affecting SEAP activity.

 

4). Reagent Preparation

Equilibrate UA-Blue detection reagent to room temperature (RT) before use.

 

Buffer Handling (40×  Buffer)

May appear slightly turbid (no precipitate) - vortex well before dilution.

Storage: 4°C: Stable for 2 weeks; -20°C: Long-term storage

 

Substrate Handling (250× Substrate)

Appearance: Red-brown solution; precipitate may form during storage.

To resolubilize: Vortex vigorously for 1–2 min, OR Incubate at 37°C for 5–10 min.

Storage: 4°C: Stable for 4 weeks; -20°C: Long-term storage

 

5). 1× Working Solution Preparation

Example (40 mL 1× detection reagent):

- Dilute 1 mL 40× Buffer in 39 mL sterile water → 1× QB Buffer.

- Add 167 µL 250× Substrate → immediate flocculation occurs. Vortex immediately until fully dissolved (clear solution). Delay in mixing causes irreversible precipitation.

 

6). HTS Optimization (e.g., 1536-well plates):

Prepare 3× or 6× concentrated detection reagent to minimize pipetting steps. Example:

3× reagent (2 µL) + 4 µL sample → 1× final concentration.

 

Storage for 1x/3x/6x detection reagents: 4°C, stable for 1 week (may form slight precipitate; vortex before use).

 

7). Assay Setup (96-Well Plate Example)

- Add 20 µL sample/control per well.

- Add 180 µL 1× detection reagent.

- Mix on plate shaker (15 sec).

 

8). Incubation: 37°C for 30 min-6 hrs (protect from light).

 

9). Detection: Measure absorbance at 600-650 nm (OD) using a plate reader.

Guidelines

Signal Stability: Readings should be taken within 6 hr of reagent addition.

HTS Adaptation: For 384/1536-well plates, scale down volumes proportionally.

Interference Control: Heat pretreatment (65°C) is recommended for high-background samples.

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SKU: 63137015094

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Theo
Lake Worth, US
★★★★★ 5
Check the height of your keyboard before ordering
This is a beautifully made, elegant looking wrist rest. The finish is satin perfectly smooth to the touch. As keyboards vary in height, be sure to measure he height of the front edge of yours before ordering—I didn’t (which is entirely my fault) and found that this wrist rest is just a wee bit too tall, which positioned my wrists a tad higher than I’m used to. Another factor you may want to consider are the ergonomics of solid wood versus other materials and your personal preferences (e.g., I tend to prefer cloth covered gel, because he cloth keeps my wrists from sticking and the gel is cushioning). My closing thought: this wrist rest is that it is by far the best looking of any wrist rest I’ve ever seen. [Disclosure: I received this product for “free”—in quotes because the IRS considers the item’s retail value as taxable income for federal income tax purposes. This review is my personally written—not AI generated—honest opinion, and is uncensored by the seller.]
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Reviewed in the United States on July 29, 2025
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Verified Purchase
Reviewer MHM 3-5
Fort Morgan, US
★★★★★ 5
Simply outstanding.
Color: Walnut, Size: 42.5 Inch, Color: Walnut, Size: 42.5 Inch
For years I was against monitor risers and stands as a concept easily defeated by VESA mount on monitor arms, but I overlooked a secondary function that monitor risers have: Display and storage. I have too many keyboards. Three that I regularly use. Now I can throw two of them in a drawer when not in use, or I can look at them and appreciate them all the time. I’m not a fan the usual keyboard displays either: awkward acrylic stepladders that they are. So had to figure out something else. I also have a headphone amplifier that I like quite a bit but was just sitting out on the desktop. So I looked at seemingly every monitor riser that exists on Amazon, and elsewhere and found that this Fenge product (Rodin on their website) fits the intersection of value, good build quality, and beautiful design aesthetic. Unlike the Hexcal shelf, which has great build quality and design, but costs $600+, for $109 (at time of purchase), you get a visually intriguing multilevel riser with aluminum hairpin feet, good sturdy materials, a beautiful (albeit synthetic) warm walnut finish (though available in four other finishes as well). Cleans easily with a dry or lightly damp rag. This isn’t your Mind Reader riser with all function but being an eye sore. It’s also pricey enough and has an interesting enough product to feel premium without being unreasonably priced for what you get. Building it was a pleasure. Packed so well, and everything in order as far as parts go. Wasn’t missing any, assembled so easily, and even better to see in person than in pictures. I have a 72” desk and had to rearrange some things to fit it, but well worth it. It serves the functions I need, but if you need more from it (like holding your devices), there’s another variant with phone, headphone and laptop stand built into this design. Also, if this isn’t visually interesting enough, there’s a third version with RGB and an acrylic lower shelf to create some illuminating pop on your workspace. As you can see, my workspace has no shortage of illumination as is, so I skipped that. But I think it did well. It partially displays my keyboard collection, stores my headphone amp, can hold a pen or two as I study, and I don’t need it to do much more on the regular. I know it won’t fit every desk aesthetic, but if you already have tech statement pieces on your desk, this may be the perfect complement.
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Reviewed in the United States on August 22, 2025
L
Verified Purchase
Lakes
New York, US
★★★★★ 5
Well made and stylish riser for my sit stand desk.
Color: White, Size: 42.5 Inch
I got this to provide a place to tuck my 49 key Arturia keyboard. The only thing I wish they would offer is a taller leg option by 1 or 2 inches in height. Regardless this thing is a beast and will easily hold a wide screen monitor or dual 27” monitors and space to tuck your laptop underneath on the middle shelf out of the way. I happen to have my monitor on an arm to provide even more usable space on the top. I have a USB switch on the right middle shelf and room for my AirPods on the left with room to spare. My Apple pencils fit perfectly on the tray as well as my iPad. In order to make this work for my application I ended up getting some acrylic cubes and silicone bumpers and pads to raise the height by two inches so my 49 key keyboard would easily fit underneath. It turned out really well for the look I wanted. If the legs were longer I wouldn’t have had to take it this far. The color is pure white and matches my desk color perfectly. It was super easy to put together. It is very solid and has a durable easy to clean finish. All in all I have no regrets making this purchase. It is very functional even without the height modifications that were specific to my needs. I will come back with a photo or two once I get settled in my new place and finish getting my studio set back up.
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Reviewed in the United States on August 3, 2025
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Noah
Bozeman, US
★★★★★ 5
i’m in loveeeeee
Color: White, Size: 42.5 Inch, Color: White, Size: 42.5 Inch
I’m not a person who often writes product review but this time i’ve decided to write one on this dual monitor stand i just got because it is AMAZING. Here’s what I love about it: - Easy to assemble - Smart and sleek design, I must emphasize on this omg it looks so stable - Space-saving Really recommend this for it’s quality and great design, one of my best amazon finds 🫶
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Reviewed in the United States on April 4, 2026
M
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Momo
Lexington, US
★★★★★ 5
Saved my neck and my desk space! Solid and stylish
Color: Walnut, Size: 42.5 Inch
I’ve been looking for a way to raise my dual 27-inch monitors, and this Fenge stand is perfect. The 42.5-inch length is exactly what I needed—both monitors fit with room to spare. It’s raised them to the perfect ergonomic height, so no more neck strain! The wood finish looks much more expensive than it actually is, and the storage space underneath is a lifesaver for hiding my docking station and keyboard when I'm done for the day. It feels very sturdy and doesn't bow in the middle at all. If you want a clean, organized desk, this is the way to go.
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Reviewed in the United States on February 9, 2026

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