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Description
Human SHBG ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Sex Hormone Binding Globulin (SHBG) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Sex Hormone Binding Globulin (SHBG) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Sex Hormone Binding Globulin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Sex hormone-binding globulin (SHBG), or sex steroid-binding globulin (SSBG), is a glycoprotein that binds androgens and estrogens. Other steroid hormones, such as progesterone, cortisol, and other corticosteroids, are bound by SHBG. SHBG is present in all vertebrates except birds. Testosterone and estradiol circulate in the blood, primarily loosely bound to serum albumin (approximately 54%) and, to a lesser extent, tightly bound to SHBG (approximately 44%). Only a tiny fraction, approximately 1-2%, remains unbound, or "free," and is therefore biologically active, able to enter cells and activate their receptors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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★★★★★ 5
Amazing how fast it works
Item Package Quantity: 1
This works so much better than the prescription cream I got from the doctor. I thought 40% was the highest available. Ease of use and it goes exactly where it's needed. Saw improvements after the second day of use.
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Reviewed in the United States on April 4, 2026
★★★★★ 5
Amazing cream to restore cracked skin!!
Item Package Quantity: 1
Cracking of my feet is not the problem. This has been the only cream I found that restores my cuticles!! My right thumb constantly stays dry and ugly. The convenience of applying like a ChapStick is wonderful. and allows me to grab and apply frequently. My cuticle has finally become soft and pretty. :) (Highly recommend)
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Reviewed in the United States on December 11, 2025
★★★★★ 5
Absolutely great. I’m a repeat buyer.
Absolutely LOVE this toner. My skin is slightly sensitive and this formula NEVER burns my face. Great exfoliation. No tightness or dry areas. Fantastic for the price and just as good or better than name brand or expensive toners. I’m a repeat buyer. Big bottle and lasts a while. I would highly recommend.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Hard to keep on the shelf
Yesyesyesyes
Imo it's the perfect male equivalent of a Honeypot wipe. It's also rock solid for drying out oily skin but to me it's kind of expensive to use it like that. Easily my favorite out of all the Thayers tonics. The serum is also really good for converting red patches into poppable pimples which then just leave, and the sulfur cream is just invaluable as a leave-on make-up/healing combo. Thayers is good stuff man. It's a billion times healthier than benzoyl peroxide or adapalene and even SA is pretty rough on the skin in therapeutic doses. Thayers does such a good job of balancing herbs and gentle chemicals to provide a uniquely safe yet effective remedy, across the board. The only thing that was a genuine flop from them for me was the rosewater tonic, which smelled like God.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Results!!
I have been using this product daily for just over two years. It’s one of those things that you just put into your daily routine and sort of forget about it.
I usually don’t expect to see a huge improvement with any product. I just use them hoping that I will maintain the status quo and that things won’t get too much worse. :)
This past Mother’s Day, I was with my sons and grandkids. I usually avoid letting them take my picture because these darn kids always seem to catch me with an unflattering look on my face. I’m pretty sure they do this on purpose. Anyway, I must not have had my guard up quite enough because when I got home, my phone alerted, and it was pictures, of me, taken incognito. 😎
I cautiously looked, and to my surprise I couldn’t believe how good the skin on my face looked!
I hurriedly scrolled back in my pictures on my phone to find a “before” picture from three years ago and I couldn’t believe my eyes. My skin looks so much better now than it did three years ago.
I wondered, how could this be? The only change that I made was adding this product to my routine a little over two years ago. There is an actual noticeable difference.
Now, I realize that my experience is nowhere near proof beyond all doubt, however, I’m not taking any chances. I will continue to use this product daily.
It’s relatively inexpensive and my results have been great.
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Reviewed in the United States on May 15, 2026
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