Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 23 - Jul 28
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human FOXP3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Forkhead Box Protein P3 (FOXP3). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Forkhead Box Protein P3 (FOXP3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Forkhead Box Protein P3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. As a member of the FOX protein family, FOXP3 appears to play a master role in regulating pathways involved in the development and function of regulatory T cells. In cancer, excessive regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune diseases, insufficient regulatory T cell activity allows other autoimmune cells to attack the body's own tissues. While the precise mechanisms of control remain unknown, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are hypothesized to exert control during transcription through similar DNA-binding interactions. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 28 reviews
Sort
Product Reviews
★★★★★ 5
Longtime Berkshire Buyers 🩶
Color: Stormy Weather, Size: King (90 in x 108 in)
We have been Berkshire buyers for years and they never disappoint with their quality. They always make the best bedding on the market at a reasonable price point. In the economy we currently live in, it's getting harder to find quality products that don't break the bank & Berkshire has been consistent for years!
This blanket is no different & has held up to their name and brand. This blanket is large, which I love. There's nothing more annoying than trying to cover your whole body with a 50x60 sized blanket lol. This blanket is most definitely aesthetically pleasing but also keeps us warm. It feels like this blanket traps our own body heat and keeps it contained instead of producing heat, making us hot. We always stay a nice, consistent temp without getting overheated and sweaty....nobody wants that lol. It's made from high-quality fabric that isn't only soft and comfy but it's woven tightly and has held up wonderfully for how much we use it! This blankey also washes really well ((i.e. zero shrinkage, dries in an adequate time, no color fading and/or bleeding)). I also appreciate that even though this blanket keeps us warm, it isn't heavy & pretty lightweight compared to some of our other non Berkshire blankets. The color is also a rich blueish, cool colored gray that accentuates all of our decor and other bedding.
Overall, Berkshire has always been a household name in ours. We love the quality, the consistency of knowing what we're getting when we purchase from their brand, and the prices are always crazy reasonable without sacrificing what you receive. Hands down another great item from their brand & we will always recommend ✔️
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 25, 2026
★★★★★ 5
Soft, plush, lovely
Color: Blue Moment, Size: King (90 in x 108 in)
We got this blanket in king size to use in lieu of a comforter in the warmer months. It's incredibly soft and luxurious. It's thick enough to feel plush and heavy, but thin enough to be perfect for a cool summer night.
It feels like really excellent quality, too. This is not cheap thin fleece. I've only washed it once so far, but it seems like it'll hold up to many washes. It's big, though, at least in king size. It takes up pretty much our entire washing machine, so it needs to be washed by itself or with little else. To dry, I tossed it in the dryer for 40 minutes or so on medium heat and then hung it over a banister to finish drying, and this seemed to work well.
It's also a very pretty blue, like a dusky light blue, just lovely. Overall, I'm very happy with this blanket.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 4
A good lightweight blanket
Color: Blue Moment, Size: Twin (60 in x 90 in), Color: Blue Moment, Size: Twin (60 in x 90 in)
With perpetually dry hands I have issues with certain fabric textures. This Velvetloft blanket is on the border of triggering, but nothing like how the Classic Extra-Fluffy Plush throw is. The ribbing is interesting visually, but also made the blanket feel a lot lighter too. Definitely one I'll keep on the couch for a chilly day and not for my bed.
When I washed it I was expecting a lot of shedding like the Classic one, but there wasn't as much. I washed it on cold and dried it on the delicate setting since I don't have a good place to air dry. It doesn't appear any worse off than before I washed it.
Overall I like this one way more than the Classic, but the Primalush wearable blanket is still my favorite.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 28, 2026
★★★★★ 5
Wait until you unroll this beautifully soft blanket! It’s the one you want.
Color: Goose Egg, Size: Twin (60 in x 90 in), Color: Goose Egg, Size: Twin (60 in x 90 in)
I can’t pass up a good blanket, and boy is this one impressive! It’s another high-quality product from Berkshire Home Company.
What I love about my goose-egg colored, velvety soft, blanket is the ribbed design. The lines separate the elevated design, and I could literally rub my hand over it throughout an entire movie!
It’s SO plush and soft. It’s made from their signature VelvetLoft fabric. Plus the design makes it look like a planned piece of your decor. I like the color I chose but there are others to choose from as well.
It’s nice and thick but not too warm. I got the 60”x90” twin size which is great for me! Next time I will get a queen size to go over my bed.
The price point is so low for the quality you get! You should stop your search now because this is the best one!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Luxurious VelvetLoft From Berkshire
Color: Stormy Weather, Size: Full/Queen (90 in x 90 in)
Berkshire hits it out of the park with yet another fabulously lux blanket. Their VelvetLoft is my favorite for warmth and softness all while looking like an offering at a high end hotel or B&B.
The medium weight was the perfect choice to pair with the large ribbing and while this IS the blanket to choose once the leaves start to fall, it's almost sinful to snuggle up with to watch a movie with the AC humming in the warmer months since it washes like a dream and did not shed at all.
I used cold, delicate and my favorite scented delicate wash. Dryer on Medium for 10 minutes and then Low for anther 20 minutes. Perfect!
The Stormy Weather is a silvery gray neutral and will work with multiple coverlets, bedspreads and quilts.
Perfect gift! Definitelythe blanket you want in the guest room!.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2026