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Description
Human SHBG ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Sex Hormone Binding Globulin (SHBG) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Sex Hormone Binding Globulin (SHBG) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Sex Hormone Binding Globulin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Sex hormone-binding globulin (SHBG), or sex steroid-binding globulin (SSBG), is a glycoprotein that binds androgens and estrogens. Other steroid hormones, such as progesterone, cortisol, and other corticosteroids, are bound by SHBG. SHBG is present in all vertebrates except birds. Testosterone and estradiol circulate in the blood, primarily loosely bound to serum albumin (approximately 54%) and, to a lesser extent, tightly bound to SHBG (approximately 44%). Only a tiny fraction, approximately 1-2%, remains unbound, or "free," and is therefore biologically active, able to enter cells and activate their receptors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.2 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
Beautiful
Size: 18-22 1.5L
I love it
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 5
Great quality Cabin and air filter bundle pack. Very solid quality.
Size: 18-22 1.5L, Size: 18-22 1.5L
First off, I like that it's a combo pack instead of me having to buy each one individually and being able to change both at the same time. Now, I must say the quality of both of them is fantastic; I'm really impressed. It's not made out of cheap junk; it feels solid and seems like it will do a good job filtering out all the debris and dirt. It was extremely easy to install and fit perfectly like an OEM air filter and cabin air filter on my Honda Accord. The cabin air filter is definitely a solid piece, and you don't have to worry about it getting crushed or deforming as it's padded, I would assume the reason for the padding on the side of the cabin air filter is that no debris or dust sneaks through which gives it a snug fit which is very smart honestly. The same applies to the air filter that goes under the hood; it definitely feels like a quality piece.
A quality filter definitely keeps outside odors and smells from coming inside the cabin, allowing you to get fresh, clean air when you turn your AC on.
Overall, definitely a solid purchase, and I will definitely be back to buy this exact bundle pack in the future as it's well-built and fits like a glove. So, you're definitely getting great value for your money with this specific engine and cabin air filter combo pack.
There are a lot of garbage ones out there, so if it were flimsy and felt like a cheap filter, I would be giving this one or two stars. But I'm actually quite impressed with this company, and it seems to be doing a fine job. My AC does work better after installing these new filters, and of course, the car runs better with the air filter installed.
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Reviewed in the United States on April 24, 2026
★★★★★ 5
Good Fit & Quality
Size: 14-22 2.0L hybrid, Size: 14-22 2.0L hybrid
This set which includes both the engine and cabit air filters are of good quality and fits my 2020 Honda Accord Hybrid well. The engine filter feels sturdy and well built. The cabin filter, although without charcoal infusion, has many folds which is more important when it comes to effective filtering of particles. The installation was easy and for the set, this set offers a good value.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Perfect fit!
Size: 14-22 2.0L hybrid, Size: 14-22 2.0L hybrid
This is a good value for a combo package which includes a cabin air filter as well as the engine air filter. Both filters were a perfect fit in my 2019 Honda Accord hybrid. The cabin air filter airflow direction is clearly labeled and the pleats are sturdy. Installation was super easy- just drop the glove box cover, pop the cabin filter cover plate, pull out the grungy old filter and pop in the new one. Easy peasy. The engine air filter is also a perfect fit. No complaints or concerns here.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
★★★★★ 5
Perfectly Fine and Inexpensive
This off-brand filter was $10 cheaper than the local auto store, nearly $20 cheaper than the dealership, and meets the original OEM requirements. My husband is an ASE certified master technician and does all of our car maintenance and repair work himself and purchasing this filter rather than getting the filter from the dealership or the local auto store saved us a good bit of money and it is the same quality. Just make sure that the OEM number is correct for your vehicle and you have nothing to worry about.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 14, 2025
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