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Description
Human IRF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Interferon Regulatory Factor 1 (IRF1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interferon Regulatory Factor 1 (IRF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Interferon Regulatory Factor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interferon regulatory factor 1 (IRF1) is a protein encoded by the IRF1 gene. It is the first member of the interferon-regulated transcription factor (IRF) family. Initially described as a transcription factor that activates the expression of the cytokine interferon β, it has subsequently been shown to function as a transcriptional activator or repressor of a variety of target genes. It regulates target gene expression by binding to interferon-stimulated response elements (ISREs) in their promoters. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.1 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 2
Very Thin
Color: Dark Grey, Color: Dark Grey
Very thin, I expected them to be thicker.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
★★★★★ 4
Serious Exfoliating Power
Color: Rectangular Gunmetal Grey, Color: Rectangular Gunmetal Grey
They possess some serious power! After working outside and managing to acquire some spots of stubborn grime that even abrasive soap wasn't tackling, I was excited to test a scrubber out. I ran it beneath water until the foam had softened and rubbed some soap into it, occasionally squeezing to generate some foam. The first time I used it, it felt a bit more abrasive than I personally cared for, but it felt softer the second time and was more comfortable. It demolished the spots of grime, I rinsed it out, squeezed as much water out, and left it to dry. It seems like the perfect size, and putting the strap around your hand makes it very comfortable to use. They do not require a lot of pressure to work, I found that gentle pressure was more than enough to clean and exfoliate. I'm not sure what to compare the material to, it's like an open cell foam that has some grit to it. I think the first point of failure will be the strap ripping off at some point with regular use. The strap feels a bit on the cheap side and is narrow, but it isn't uncomfortable to use at all! It's really just there to help you hold onto a soapy object in the shower, and give you something to hang it up by. All in all, the price is very reasonable for what you're getting and all foam will break down with use over time so they may require replacement, but they are far from single use!
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Excellent exfoliator
Color: Rectangular Gunmetal Grey
I love this product! It exfoliated my skin so well.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
HAND-SIZED EXFOLIATORS THAT LATHER WELL AND EARN A STARRING ROLE IN MY SHOWER ROUTINE
Color: Dark Grey, Color: Dark Grey
I have been using Nigerian exfoliating nets for a while. They exfoliate very well, but they are not my favorite for building up a rich lather. These solve that problem, so they have become part of my regular shower routine. I still use a net for my back as it is hard to reach, but for everywhere else these are more practical.
PACKAGING/CONTENTS:
* You get 2 round exfoliating scrubbers in sealed compressed packaging. Giftable.
EASE OF USE:
* Very simple. Soak, lather up, and scrub.
* The elastic hand strap is useful and keeps the pad secure, so there is less fumbling and dropping.
* The round shape is easy to control around tighter areas like behind the ears, collarbone, and ankles.
SIZE:
* Size is just right for hand use.
* It is compact enough to maneuver easily, but large enough to cover a decent amount of surface area with each pass.
QUALITY:
* Quality is excellent for a $9.99 two-pack.
* The exfoliating surface has enough bite to do its job, while the softer inner body keeps it from feeling harsh in the hand.
* The stitching and edge binding look solid, and the pad keeps its shape well when wet.
SOFTNESS AND EXFOLIATION:
* These are not ultra-soft fluffy bath sponges. They are exfoliators first.
* The textured outer surface does the work, and it gives a satisfying scrub without feeling absurdly aggressive.
* I would call the center comfort-oriented and the outer surface functional.
THICKNESS:
* Thickness is dialed in well for the use.
* Thick enough to feel substantial in the hand and provide spring, but not so chunky that it takes forever to rinse or dry.
DRYING TIME:
* Drying time is fast if you hang them by the loop or strap.
* They do not stay waterlogged the way some denser bath sponges can.
VALUE FOR MONEY:
* At $9.99 for a 2-pack, these are appropriately priced.
* Anticipate replacing them monthly which is a reasonable cost for the largest organ of the body.
OVERALL:
STRONG BUY. I am a convert. Will buy again.
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Reviewed in the United States on April 21, 2026
★★★★★ 1
Don't buy, look like something for a baby
Color: Dark Grey
Inaccurate description, they are tiny and thin doesn't look nothing like the pictures
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2026
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